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Description

In the second step adding NADPH and glutathione reductase results in conversion of all GSSG to GSH which reacts subsequently with DTNB and fluorescent or colorimetric substrate to be quantified

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Quantitative polymerase chain reaction (PCR) was performed in duplicates using TaqMan gene expression assays on a CFX96 Touch Real-Time PCR machine (Bio-Rad, Tokyo, Japan)